Amplifying DNA with arbitrary oligonucleotide primers.
نویسنده
چکیده
منابع مشابه
Primase-based whole genome amplification
In vitro DNA amplification methods, such as polymerase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the reaction. In vivo, primers are synthesized on-template by DNA primase. The bacteriophage T7 gene 4 protein (gp4) has both primase and helicase activities. In this study, we report the development of a primase-based Whole Genome Amplification (pWGA) method,...
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عنوان ژورنال:
- PCR methods and applications
دوره 3 2 شماره
صفحات -
تاریخ انتشار 1993